MicroRNA-33b inhibits liver cancer cell proliferation, migration and invasion via down-regulation of Fli-1 and MMP-2 protein expressions

Purpose: To study the influence of microRNA-33b (miR-33b) on liver cancer cell proliferation, migration and invasiveness, and the mechanism involved. Methods: MicroRNA-33b or Fli-1 overexpression plasmid was transfected into liver cancer (SMMC7721) cells. Cell proliferation, migration, and invasiveness were determined using cell counting kit 8 (CCK-8), scratch test, and Transwell invasion assay, respectively. The amounts of miR-33b and Fli-1 in liver cancer tissues,  paracancerous normal tissues, and miR-33b overexpression and control groups were measured using qRT-PCR, while protein concentration of matrix metalloproteinase 2 (MMP-2) was assayed using Western blotting. Results: Fli-1 protein was markedly upregulated in liver cancerous cells, relative to paracancerous normal tissues (p < 0.05). MicroRNA-33b protein expression was also significantly upregulated in miR33b overexpression group, but the corresponding Fli-1 expression was downregulated in miR-33b overexpression group, relative to control (p < 0.05). MicroRNA-33b overexpression significantly and time-dependently inhibited SMMC7721 cell proliferation and migration, but it reduced the degree of apoptosis (p < 0.05). Liver cancer (SMMC7721) cells in miR-33b overexpression group were less invasive than the control group (p < 0.05). Similarly, miR-33b overexpression significantly downregulated MMP-2 protein expression in SMMC7721cells (p < 0.05). Conclusion: Overexpression of miR-33b suppresses the proliferation, migratory and invasive potential of hepatic cancer cells via down-regulation of Fli-1 and MMP-2 protein expression. This finding may be useful in the identification of new liver cancer drugs

Tetra­imidazole­bis(trichloro­acetato)copper(II)

The title compound, [Cu(C2Cl3O2)2(C3H4N2)4], was prepared by the reaction of imidazole and trichloro­acetatocopper(II). The CuII atom adopts a distorted octa­hedral coordination geometry, binding the N atoms of four imidazole ligands and the carboxyl­ate O atoms of two trichloro­acetate anions. The mol­ecular structure and packing are stabilized by N—H⋯O hydrogen-bonding inter­actions. Close inter­molecular Cl⋯Cl contacts [3.498 (3) Å] are also found in the structure

Hash-based Multivariate Public Key Cryptosystems

Many efficient attacks have appeared in recent years, which have led to serious blow for the traditional multivariate public key cryptosystems. For example, the signature scheme SFLASH was broken by Dubois et al. at CRYPTO\u2707, and the Square signature (or encryption) scheme by Billet et al. at ASIACRYPTO\u2709. Most multivariate schemes known so far are insecure, except maybe the sigature schemes UOV and HFEv-. Following these new developments, it seems that the general design principle of multivariate schemes has been seriously questioned, and there is a rather pressing desire to find new trapdoor construction or mathematical tools and ideal. In this paper, we introduce the hash authentication techniques and combine with the traditional MQ-trapdoors to propose a novel hash-based multivariate public key cryptosystems. The resulting scheme, called EMC (Extended Multivariate Cryptosystem), can also be seen as a novel hash-based cryptosystems like Merkle tree signature. And it offers the double security protection for signing or encrypting. By the our analysis, we can construct the secure and efficient not only signature scheme but also encryption scheme by using the EMC scheme combined some modification methods summarized by Wolf. And thus we present two new schems: EMC signature scheme (with the Minus method ``- ) and EMC encryption scheme (with the Plus method ``+ ). In addition, we also propose a reduced scheme of the EMC signature scheme (a light-weight signature scheme). Precise complexity estimates for these schemes are provided, but their security proofs in the random oracle model are still an open problem

Effect of Physical Exercise on Life Satisfaction of Chinese Primary Students: The Chain Mediating Role of Self-Confidence and Resilience

Life satisfaction is an overall cognitive evaluation of an individual\u27s living condition for the most of time or over a certain period of time according to the standard of one’s own choice. It is an important psychological variable in the developmental stage of children and adolescents. Some studies had shown that life satisfaction was closely related to children\u27s and adolescents\u27 mental health levels, including their emotional and behavioral conditions. However, the Blue Book for Children: China Children\u27s Development Report (2020) published by China Children\u27s Center stated that Chinese students did not fare well in terms of life satisfaction. Previous studies had indicated that physical exercise was an important factor that had a positive effect on life satisfaction, as well as on self-confidence and resilience. Also, there is a correlation between self-confidence, resilience, and life satisfaction. Nonetheless, few studies have researched the role of self-confidence and resilience in the impact of physical exercise on the life satisfaction of primary school students. To explore the mediating role of self-confidence and resilience between physical exercise and life satisfaction, in order to provide a theoretical basis and practical guidance for how to effectively promote life satisfaction and psychological well-being of primary school students in the practice of physical exercise. Group measurements of 1009 students (519 boys and 490 girls; 576 fifth graders and 433 sixth graders) are carried out by using the Scale of Physical Exercise Level, Children and Adolescent Self-Confidence Scale, Children and Adolescent Life Satisfaction Scale, and Adolescent Resilience Scale, and Structural Equation Model and Bootstrap are used to analyze the mediating effect of self-confidence and resilience. The results reveal that there is a positive correlation between physical exercise and life satisfaction (r=0.218), but the effect on life satisfaction is not significant (P=0.516); the indirect effect of physical exercise on life satisfaction consists of two paths: physical exercise to self-confidence to life satisfaction (95% CI: 0.128,0.267); physical exercise to self-confidence to resilience to life satisfaction (95% CI:0.109,0.209). It is concluded that physical exercise has an indirect but significant effect on primary school students\u27 life satisfaction--the independent mediating effect of self-confidence and the chain mediation effect of self-confidence and resilience. The study further confirms that physical exercise can promote students\u27 self-confidence levels and the formation of tenacious psychological quality, thus improving their life satisfaction levels. Therefore, society, schools and families should pay close attention to the positive effects of physical exercise on primary school students’ emotional and behavioral conditions